Identificación y actividad farmacológica de principios de especies antiinflamatorias.
In this work we studied medicinal plants used such as anti-inflammatory agents in skin diseases in both Chinese traditional medicine and other folk medicines. The species studies were Schinus molle, Lysimachia foenum-graecum, Lithospermum erythrorhizon, Forsythia suspensa and Isodon xerophilus.
In all the cases, plant materials were extracted with the adequate solvents and fractioned with solvents of increased polarity. All the fractions were subjected to an analytical study, and the more representative of them were subjected to a pharmacological study. From the active fractions the principal compounds present were isolated and identified.
Different experimental protocols of acute and chronic inflammation were used such as the chronic and acute mouse ear oedemas induced by 12-O-tetradecanoilforbol 13-acetate de (TPA), mouse paw oedema induced by carrageenan and phospholipase A2 (PLA2). Moreover, the cytotoxicity, inhibition of nitrite production, leukotriene B4 production and peptidic mediator production in different cells, as well the antioxidant activity, were tested.
The most relevant results were: a) isolation and identification of two active triterpenes and a biflavanone from the dichloromethane extract of Schinus molle and study of their anti-inflammatory activity; b) isolation of five new saponins from Lysimachia foenum-graecum and study of their cytotoxicity and inhibition of leukotriene B4 production; c) determination of the antioxidant activity of extracts from Lithospermum erythrorhizon and principles from this species; d) isolation of two anti-inflammatory triterpenes from Forsythia suspensa and study of their antioxidant activity; and, e) isolation of fourteen diterpenoids from Isodon xerophilus and study of their cytotoxicity and inhibition on nitrite and TNF-? production.
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Advisor:Ríos Cañavate, José Luís
School:Universitat de València
Source Type:Master's Thesis
Date of Publication:09/08/2006