n vitro lymphocyte/nervous cell interactions in Trypanosoma cruzi experimental infection: a possible role for the extracellular matriz.
Chagas disease, caused by the protozoan Trypanosoma cruzi, is an endemic parasitic disease affecting more than 18 million individuals in Latin America. Part of these patientsdevelop symptoms related to the disorders in the peripheral and central nervous system. It has been described, during the acute phase of the disease, an intense inflammatoryinfiltrate in the nervous tissue, but the mechanism(s) driving T cells to this tissue remain(s) to be clarified. We used an in vitro model to study the interactions between T cells derived T. cruzi-infected mice and neurons, herein exemplified by the N2a murine neuroblastoma cell line, as well as by a brain cortex-derived primary culture of murine neurons. In particular, we looked for extracellular matrix (ECM)- mediated interactions,known to affect T cell migration. We first showed that N2a cells and the primary cultures of neurons constitutively expressthe ECM proteins, laminin, fibronectin and collagen type IV and that such an expression is upregulated upon T. cruzi infection in vitro. Moreover, adhesion of peripheral T cells was enhanced (as compared to non- infected conditions) when N2a cells were infected in vitro, or when T cells were derived from T.cruzi infected mice. This likely represents an ECM- mediated event since it could be partially inhibited with anti-ECM antibodies. In conclusion, our result s indicate that ECM ligands and receptors are involved in the T cell/neuronal interactions that may occur following T. cruzi infection.
School:Faculdades Oswaldo Cruz
Source Type:Master's Thesis
Keywords:Trypanosoma cruzi Lymphocytes Neurons Extracellular Matrix
Date of Publication:12/18/2002