Determinació d'antibiòtics en mostres biològiques per electroforesi capil·lar.
DETERMINATION OF ANTIBIOTICS IN BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS
The main objective of this Thesis is the development of analytical methods for determining several antibiotic families by capillary electrophoresis.
Other objective of this Thesis is the application of capillary electrophoresis as analytical technique for determining antibiotic residues in biological samples. These samples are very complex and for this reason we have developed pretreatment systems for extracting the compounds from the matrix and to diminish the amount of interfering substances.
In the other way, one of the most important disadvantages in capillary electrophoresis is that usually we can not determine low concentrations. For this reason another objective of this Thesis is to study several ways to decrease detection limits of capillary electrophoresis system. Thus we have studied several on-column preconcentration systems, such as field amplified sample injection and capillary isotacophoresis. These systems are able to increase rapidly and simply the sensibility of the system several magnitude orders.
By this way in this Thesis we have developed several analytical methods for determining several antibiotics and antibiotic families in different types of biological samples. We have studied the following antibiotic families: penicillins, quinolones, tetracyclines and aminoglycosides. Moreover, they have been analysed in several biological samples, such as plasma and different tissue samples (e.g. liver, kidney and muscle). All of these studies have been developed in animal samples except in one of them in which it was developed in human serum.
This Doctoral Thesis is structured in four chapters. The first one includes an introduction in which is explained the antibiotic concept and is described the legislation which controls the administration of antibiotics in animals, and explains the necessity of developing analytical methods to determine residues at low levels in biological samples.
The second one includes a review about several papers in which are developed the analysis of antibiotics in biological samples. There is described which are the systems more used in this kind of studies and the use of capillary electrophoresis as a real and suitable alternative. Moreover, this chapter includes a copy of work which analyzed antibiotics in biological samples by capillary electrophoresis. This work will be published in an international journal, Trends in Analytical Chemistry.
The third chapter includes the experimental part of this Thesis. By this way, this chapter includes the eight works published in international journals (Electrophoresis, Journal of Chromatography B and Chromatographia). Before each paper there is included a brief explanation about the paper contents. Some of published papers are based in the study of several types of pretreatment systems. And the other works include comparative studies to determine which is the best system to increase system sensitivity.
The last chapter describes the conclusions obtained through these works.
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Advisor:Calull Blanch, Marta; Borrull Ballarin, Francesc
School:Universitat Rovira i Virgili
Source Type:Master's Thesis
Keywords:departament de química analítica i orgànica
Date of Publication:12/18/2002