Caracterització, quantificació i persistència en plantació del fong ectomicorrízic comestible Lactarius deliciosus en sistemes de producció controlada
The main objective of the thesis project is the production of inoculated plants with selected strains of the edible ectomycorrhizal fungus Lactarius deliciosus and tracking its persistence once established in field plantations. The colonization ability of 25 strains of L. deliciosus has been evaluated in pure culture synthesis with Pinus pinaster. The results have shown differences in root colonization level of the evaluated strains. The formation of mycorrhizas has been negatively related to the age of the fungal culture and positively to the formation of rhizomorphs. Different molecular techniques have been optimized for specific and intraspecific characterization of L. deliciosus and evaluated for their effectivity in field identification of the fungus in the cryptic phases of the symbiosis (mycorrhizas and extraradical mycelium present in the soil). The design of the primer named LDITS2R in the ITS region of the rDNA of L. deliciosus combined with the universal primer ITS1 has allowed specific amplification of this fungus by PCR from DNA extracted from field samples. Single strain confromation polymorphisms (SSCP) analysis of the specific amplified products has provided nine different patterns among the 18 L. deliciosus analyzed strains which allowed for the intraspecific identification of the selected inoculated strain. Plants mycorrhizal with L. deliciosus have been produced in the nursery and initial radical colonization has been individually quantified in a non-destuctive way by analysis of digital photographs. Experimental plantations have been established with the inoculated plants. Fungal persistence has been evaluated at different times by determining the percentage of mycorrhizas and the production of extraradical soil mycelium by real time PCR. The site and the inoculated strain significantly affected the persistence of mycorrhizas and the extension of the extraradical soil mycelium whereas no effect of the initial colonization level was observed under the experimental conditions tested. Finally, the persistence of L. deliciosus against two Rhizopogon species identified as potential competitors has been evaluated. The presence of R. roseolus in the field site affected negatively the persistence of L. deliciosus. The results obtained suggest that competition between these two fungi takes place at the root system level for colonization of the available roots. No negative interaction between extraradical mycelia of both species was observed. Linear correlations between the percentage of mycorrhizas and soil mycelium biomass were significant and positive for the three fungal species studied. Consequently, the quantification of extraradical mycelium could be a good indicator of the root colonization level of the plant allowing for non-destructive tracking of the fungus in the field.
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Advisor:Santamaria del Campo, Sergi; Parladé Izquierdo, Xavier
School:Universitat Autónoma de Barcelona
Source Type:Master's Thesis
Keywords:407 departament de biologia animal vegetal i ecologia
Date of Publication:04/21/2008