Avaliação do processo de descontaminação de brocas odontológicas e seu impacto no controle de infecção

by Anders, Patrícia Staciarini

Abstract (Summary)
Objective: to describe the process of decontamination of burs to assess the microbial contamination of burs after-processing that were available to the use to isolate and to identify the possible microorganisms Methods: This study was conducted in 110 private dental offices of the central area of Goiânia-Goiás during the period of March/2004 to August/2005 using a check-list measure of dry heat sterilizer temperature and microbial burs tests The burs were seeded in brain heart infusion broth incubated at 37ºC for 20 days and subcultured on specific agar to isolate microorganisms The isolates were identified by micro/macroscopic characteristics subcultured on specific agar biochemical/enzymatic test and automation technique (MicroScan ®) Results:A total of 110 burs were evaluated and 35 (31.8%) were contaminated Fungi were detected in 13 (30.2%) burs [Aspergillus sp (27.9%) and Micelia (2.3%)]; Gram-positive cocci (staphylococci) represented 13 (30.2%) isolates [2.3% Staphylococcus aureus and 27.9% (12) coagulase negative staphylococci] nine(20.9%) isolates were Gram-positive bacilli and eight (18,6%) fastidioso microorganisms Considering the obtained results some factors detected could be interfered in the burs sterilization ineficiency: enzymatic detergent inadequate use abrasive products use inadequate dry heat sterilization timeand temperature multiple use burs kits and interruption the asseptical chain after sterilization Conclusion: The frequency of contaminated burs was high (31.8%) and it was detected failures in operational steps of burs processing and/or after sterilization
This document abstract is also available in Portuguese.
Bibliographical Information:

Advisor:Anaclara Ferreira Veiga Tipple; Fabiana Cristina Pimenta

School:Universidade Federal de Goiás

School Location:Brazil

Source Type:Master's Thesis

Keywords:articles processing decontamination crosscontamination


Date of Publication:03/31/2006

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