Expresión de los receptores de quimioquinas en células de sangre periférica y en especial en los subtipos de los monocitos en pacientes con artritis idiopática juvenil de inicio sistémico comparados con individuos sanos

by Antón López, Jordi

SUMMARY: Juvenile Idiopathic Arthritis is the most common of the rheumatic diseases in childhood. 15% of these patients present a Systemic onset Juvenile Idiopathic Arthritis (SoJIA). SoJIA is characterized by arthritis, fever, serositis, lymph node enlargement, and an increase in inflammations markers. Our hypothesis was that the expression of chemokine receptors in cellular surface of peripheral blood cells is different in patients with SoJIA compared with healthy patients. Using flux cytometry 21 patients with SoJIA were compared with 12 healthy children and adults. The results shown that the expression of chemokine receptors in peripheral blood mononuclear cells of SoJIA patients was different than in healthy subjects. There were seen differences regarding if patients were in remission or not, or if they were or not under treatment with steroids. - CCR1 expression was decreased in all the subtypes of SoJIA monocytes. - CD62-L expression was increased in granulocytes of symptomatic and asymptomatic under treatment SoJIA comparing with asymptomatic and healthy. - CXCR2 expression was increased in asymptomatic under treatment SoJIA granulocytes comparing with healthy subjects. - CCR2 expression was increased in CD14+CD16+ monocytes of asymptomatic under treatment SoJIA comparing with healthy. - The expression of CD62-L was lower in CD14+CD16- monocytes of SoJIA in remission (asymptomatic without treatment) than in healthy subjects and the rest of active SoJIA patients. That could be understood as a characteristic of SoJIA under remission. - SoJIA symptomatic presented an increased expression of CD62-L and CD64 in classic monocytes (CD14+CD16-) comparing with asymptomatic SoJIA Finally, a cell population was identified. This population presented a high CD14 and CD16 expression (CD14++CD16++).This cell population was increased in SoJIA patients.