Certificación de la concentración de masa de la isoenzima 2 de la creatina-quinasa del material de referencia BCR 608
This report describes the certification of a mass concentration value to the already prepared creatine kinase 2 reference material (BCR 608) with a catalytic concentration assigned value. At present, a mass creatine kinase 2 reference method have not been described. For this reason, we measured the BCR 608 mass concentration in different immunoassays using a creatine kinase 2 purified solution as a calibrator. Previously, the protein concentration of the creatine kinase 2 solution was measured by Doestch method using the reference material SRM 927b as calibrator. Creatine kinase 2 was purified from human heart. A protein concentration value of 124.30 mg/L was assigned to the solution of purified creatine kinase 2 by Doetsch method, and then this solution was diluted to obtain a liquid standard of known protein concentration (80.36 mg/L) to calibrate the immunoassays. The mass concentration of creatine kinase 2 in the BCR 608 reference material was certified to be 94.5 mg/L with an associated uncertainty of 9.6 mg/L.
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Advisor:Francesca Canalias; F.Javier Gella
School:Universitat Autónoma de Barcelona
Source Type:Master's Thesis
Keywords:406 departament de bioquimica i biologia molecular
Date of Publication:03/15/2001