Distribuição de neurônios imunorreativos à triptofano hidroxilase no tronco encefálico e diencéfalo de pombos (Columba livia) e sua co-localização com serotonina

by Meneghelli, Cristiane

Abstract (Summary)
The distribution of tryptophan hydroxylase (TPH)-containing perikarya and processes in the brainstem and diencephalon of the pigeon (Columba livia) was investigated using single-labeling chromogenic and double-labeling fluorescence immunohistochemical methods for TPH and 5-HT. TPH-immunoreactive perikarya were seen extending from the caudal medulla to mid-hypothalamic levels, located in brainstem regions previously described as containing 5-HT-immunoreactive somata. Brainstem TPH-immunoreactive cell clusters (the midline raphe, and the dorsolateral and ventrolateral cell groups) and the circumventricular cerebrospinal fluid-contacting neurons in the taenia choroidea (in the caudal brainstem), recessus infundibuli and paraventricular organ (in the hypothalamus) were shown to coexpress 5-HT immunoreactivity. However, heavily labeled TPH-immunoreactive cell clusters were observed in the nucleus premamillaris, in the stratum cellulare internum, in the nucleus paraventricularis magnocellularis and in the medial border of the nucleus dorsomedialis anterior thalami. Double-labeling experiments indicated that all of these hypothalamic TPH-immunoreactive cells were not immunoreactive to 5-HT. These cells correspond to dopamine- and melatonin-containing neurons previously found in the avian hypothalamus, and appear to be comparable to the mammalian TPH-immunoreactive hypothalamic A11-A13 catecholaminergic somata, suggesting that they may be a conserved attribute in the amniote hypothalamus
This document abstract is also available in Portuguese.
Bibliographical Information:

Advisor:José Marino Neto; Mariana Graciela Terenzi; Cilene Lino de Oliveira; Jackson Cioni Bittencourt

School:Universidade Federal de Santa Catarina

School Location:Brazil

Source Type:Master's Thesis

Keywords: Hipotalamo


Date of Publication:02/10/2009

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