Caracterização de genes de resistência a patógenos em eucalipto (Eucalyptus ssp.), cana-de-açúcar (Saccharumssp.) e feijão-caupi (Vigna unguiculata)

by Wanderley Nogueira, Ana Carolina

Abstract (Summary)
Resistance genes (R) are responsible for the first plant-pathogen interaction, being responsible for activation of resistance pathways is plants. The present work analyzes R genes in sugarcane, eucalyptus and Vigna unguiculata expressed sequence tags, generated by libraries produced from different tissues and development stages. After in silico screening was possible to identify all R- genes classes in sugarcane, specially KINASE (that presented 46% of 196 candidate sequences with conserved domains), in eucalyptus the most abundant was NBS-LRR ( Nucleotide Binding Site- Leucine Rich Repeats) that presented 50% of 208 candidates sequences with conserved domains. Despite the available Vigna unguiculata sequence numbers was scarce, the most abundant class was NBS-LRR (80% of the 38 candidate sequences), however were absent KINASE and LRR-KINASE lasses. Could be observed R- genes in all analyzed sugarcane and eucalyptus tissues, in different expression levels under non induced conditions. When analyzed trough multiple alignments, R-genes presented more similarities among species that pertain to the same family, generally, grouping monocots and dycots in different branches, suggesting their appearing before the separation between these classes. Our result have potential to collaborate to molecular markers developing, with best understanding of these genes diversity and evolution, not only in these studied species, but already, in other economic interests crops
This document abstract is also available in Portuguese.
Bibliographical Information:

Advisor:Ana Maria Benko Iseppon

School:Universidade Federal de Pernambuco

School Location:Brazil

Source Type:Master's Thesis

Keywords:Fitopathology Resistance genes- Eucalyptus Sugarcane Vigna unguiculata


Date of Publication:07/09/2007

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