Doenças de Newcastle: padronização de testes sorológicos para o diagnostico em avestruzes (Struthio Camelus) e avaliação soroepidemiológica nos Estados da Bahia e de São Paulo
Newcastle Disease is a highly contagious viral infection, which attacks severalavian species, and represents one of the most important diseases for the modernpoultry industry. Because of the relevance of the poultry industry in Brazil and theboom in ostrich rearing, it?s necessary the development of control strategies and itdepends on the development of practical and reliable tools for diagnosis. Suchtools are available for chickens; however specific tests were not developed forostriches yet. The aim of the present work is to standardize serological tests forthe detection of antibodies against the Newcastle disease in ostriches and assessthe seroepidemiological situation of flocks located in Bahia and São Paulo. Thestandardization of haemagglutination inhibition (HI) revealed interference of thetype of used red blood cells and demonstrated the need of the use of erythrocytesof the same species or, alternatively, of turkeys. Indirect ELISA were developed ormodified for this species and, in spite of they present high correlation amongstthemselves, they demonstrated low correlation with HI. "Western blot" and "dotblot"were developed, and that can be useful in evaluation of the immune responseand to make possible the implantation of control programs. The samples analyzedrevealed the presence of antibodies against NDV and, as these animals were notvaccinated, the hypothesis that the ostriches are in contact with the vaccine virusor wild virus is reinforced.
Advisor:Luciano Doretto Júnior; Antonio Carlos Paulillo; Nilce Maria Soares Queiroz Gama; Ruben Pablo Schocken-Iturrino; Elizabeth Santin; Songeli Menezes Freire
School:Universidade Federal da Bahia
Source Type:Master's Thesis
Date of Publication:12/15/2006