Caracterização de genótipos G de rotavírus do grupo A circulantes no Distrito Federal
Rotaviruses are characterized by a high genetic diversity. Such diversity has potentialimplication on the epidemiology of acute infectious diarrhea. This work aimed to describe thevariability of G genotypes from group A rotaviruses circulating in the Federal District, CentralBrazil. One hundred and three fecal samples kindly provided by the Public Health Laboratory ofthe Federal District were analyzed. The samples were collected at three distinct timeframes:1986 to 1990, 1994 to 1996 and 2004 to 3005. The rotavirus detection was initially performed byEIARA and PAGE. The results from these two procedures presented 98% of agreement. Onlytwo samples were positive by PAGE and non reactive by EIARA. All samples showedelectrophoretic profile compatible with group A rotaviruses. From the 103 samples analyzed, 94showed long electropherotype, whereas eight showed short and one atypical electropherotype.The extracted RNAs were reversely transcribed and amplified by PCR, using 9con1 and 9con2primers, generating DNA fragments with approximately 904 bp corresponding to part of the VP7protein gene. The PCR products were submitted to automated sequencing and the generatedsequences were analyzed by the FASTA and BLAST programs. When the three seasons wereevaluated, the predominant genotype was G1 (50.48%), followed by G9 (18.45%), G2 (10.68%),G5 (10.68%), G3 (5.83%) and G4 (3.88%) genotypes. In the first season (1986-1990), 41.18%of the samples were described as genotype G1; 23.53% genotype G5; 17.65 G3 and 8.82% G2and G4. During the second season (1994-1996), 74.47% of the samples were characterized asG1; 17.02% G2; 6.38% G5 and 2.13% G4. The most prevalent genotype of the third season(2004-2005) was G9 (86.36%) followed by G1 (13.64%). Phylogenetic analysis was performedby the neighbor-joining method and the segregation of the genotypes confirmed the homologyresults obtained by FASTA and BLAST. The information generated by this study is inagreement with the literature and may subsidize future evaluation of vaccine efficacy as well asthe monitoring of rotavirus genotypes in the Federal District and in Brazil.
Advisor:Jose Paulo Gagliardi Leite; Loreny Gimenes Giugliano; Cláudia Renata Fernandes Martins
School:Universidade de Brasília
Source Type:Master's Thesis
Keywords:rotavírus diarréia genótipos ciencias da saude
Date of Publication:07/25/2006