Correlação da morfometria e da compactação da cromatina de espermatozóides de touro Zebuíno sobre a taxa de clivagem e formação de blastocistos em programas de produção "IN VITRO"
38 PIVs carried through in two laboratories of the Uberaba ? MGBRASILhad been used in this work with the objective to evaluate the relationshipbetween condensation chromatin and head morphometry of spermatozoa andcleavages and blastocyst formation rates using semen of zebu bulls with normalroutine tests of fertility. Smears of semen stained with of Toluidine Blue and computerimage analysis had been used to evaluate the morphometry and chromatincondensation. Area (A), perimeter (P), width (L), length (C), width:length ratio (L/C),ellipticity (E), shape factor (FF), side symmetry (SL), anterior-posterior symmetry(SAP) and the three first Fourier values (F0, F1, F2) had been evaluated in themorphometric analysis. The chromatin was evaluated in relation to intensity ofcondensation and homogeneity. The results allowed concluding that the influence oflight variations in the sperm head morphometry and the chromatin condensation onthe cleavage and number of normal morfologic blastocyst in the PIV is small.
Advisor:Jose Octavio Jacomini; Marcos Silva; Marcelo Emílio Beletti
School:Universidade Federal de Uberlândia
Source Type:Master's Thesis
?in vitro? production
Date of Publication:12/20/2006