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Avaliação da atividade terapêutica do Melxi® em modelo animal de doença inflamatória do trato respiratório

by Bertão, Humberto Gonçalves

Abstract (Summary)
Melxi® is a phytotherapeutic product made from bee honey and pineapple extract (Ananas comosus L.). Honey has anti-septic and bactericidal properties, and consists of 78% carbohydrates, 1.5% proteins and 0.18% mineral salts, besides 5.72% vitamins and other elements. The raw extract of pineapple contains a complex mixture of proteases, called bromelain, in addition to other enzymes and protease inhibitors, as well as saccharose, fructose and glucose. The therapeutic efficacy of bromelain has been described in various publications, one of the most important effects being its mucolytic and fluidifying action on airway secretions. The aim of this work was to study the effect of Melxi® syrup, a phytotherapeutic product, on lung tissue and broncoalveolar lavage samples in an animal model of inflammation of the respiratory tract. Male Wistar rats were divided into four groups: Group I = Control not sensitized; Group II = Control sensitized; Group III = sensitized and treated with saline; and Group IV = sensitized and treated with Melxi®. The animals in Groups II, III and IV were pre-sensitized with 2 intraperitoneal injections (IP) of 200 amp;#956;l of 1%(w/v) ovalbumin (OVA) at intervals of 10 days, followed by sensitization with 4 intranasal applications of 50 amp;#956;l of OVA solution according to the same time interval. After sensitization, Group IV received 2 ml of Melxi® syrup by orogastric gavage, 3 times a day, while Group III received 2 ml 0.9% (w/v) NaCl under the same conditions, for 7 days. All the animals were anesthetized with pentobarbital. The lungs and broncoalveolar lavage fluid were collected and processed for 2-dimensional (2D) electrophoresis. Part of the lung was removed and fixed in 10% formalin, stained with hematoxylin/eosin, alcian blue and periodic acid Schiff, for histopathological analysis. The lung tissue of Group IV stained with PAS showed reduced amounts of intracellular deposits of glycosaminoglycans when compared with animals in Group II. The images obtained from 2-D electrophoresis gels were analyzed using the 2D Platinum software from Amersham Biosciences. The electrophoretic profiles of the proteins in broncoalveolar specimens of the different groups studied demonstrated small alterations, only in the amounts of proteins of low molecular mass in the alkaline pH region after isoelectric focusing (IEF). Meanwhile, the lung tissue specimens of the four groups were very similar. The results obtained suggest that there was a diminution in mucus production by bronchial epithelium cells in animals treated with Melxi®, but that these alterations did not appear to have influenced the lung protein profile of treated animals compared to controls
This document abstract is also available in Portuguese.
Bibliographical Information:

Advisor:José Luiz de Lima Filho

School:Universidade Federal de Pernambuco

School Location:Brazil

Source Type:Master's Thesis

Keywords:Bromelain Melxi® Proteômica Bromelina Proteomic Two dimensional electrophoresis

ISBN:

Date of Publication:02/28/2007

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