Vacina de DNA (hsp65 M. Leprae) para Paracoccidioidomicose Experimental: atividade imunogênica e terapêutica
Heat shock proteins (HSPs) are recognized as important molecules in the modulation of the immune system and are highly conserved among different organisms. The DNA-hsp65 vaccine from Mycobacterium leprae (M. leprae) has been shown to have prophylactic and immunotherapeutic effects against various diseases, for instance, tuberculosis, arthritis and leishmaniasis. In this work, we evaluated the effectiveness and immunomodulatory potential of the DNA-hsp65 immunization and treatment in model BALB/c mice infected with Paracoccidioides brasiliensis (P. brasiliensis), the etiological agent of Paracoccidioidomycosis, the most important endemic mycosis in Latin America. The DNA-hsp65 vaccine conferred protection against this pathogen for both, the prophylactic and therapeutic assays, as indicated by: a significant reduction in pulmonary fungal burden (CFUs); a decrease in pulmonary function loss and in the presence of collagen in granulomas (revealed by histological analyses of the pulmonary tissue); the reestablishment of spleen cells proliferation; the Th1 pattern a and IGg2a and lower levels of IL-4, IL-10 and immune response with higher levels of IL-12, IFN- ?, TNF-IgG1. Together, these findings indicate that, in mice, the immunization and treatment with the DNA-hsp65 vaccine protect mice against Paracoccidioidomycosis. Our results open new perspectives on prevention and treatment of other systemic mycoses.
Advisor:Andrea Queiroz Maranhao; Fernando Araripe Gonçalves Torres; Maria Sueli Soares Felipe; Ildinete Silva Pereira; Anamélia Lorenzetti Bocca; Gustavo Adolfo Sierra Romero; Carlos Pelleschi Taborda
School:Universidade de Brasília
Source Type:Master's Thesis
Keywords:DNA-hsp65 Paracoccidioides brasiliensis DNA vaccine
Date of Publication:03/12/2008