Details

USE OF FUNCTIONALIZED GOLD NANOPARTICLES TO EFFICIENTLY EXTRACT AND CONCENTRATE PEPTIDES FOR MALDI-TOF-MS DETECTION

by Vanderpuije, Benjamin P.

Abstract (Summary)
USE OF FUNCTIONALIZED GOLD NANOPARTICLES TO EFFICIENTLY EXTRACT AND CONCENTRATE PEPTIDES FOR MALDI-TOF-MS DETECTIONMAY 2007BENJAMIN PAPA KWESI NII YANKOS VANDERPUIJE, B.Sc, KWAME NKRUMAH UNIVERSITY OF SCIENCE AND TECHNOLOGY, GHANAM.S., UNIVERSITY OF MASSACHUSETTS AMHERSTDirected by: Professor Richard W. VachetWe have developed a straight forward method that uses monolayer protected clusters (MPCs) and mixed monolayer protected clusters (MMPCs) as selective extraction and concentration probes for peptides. After extraction with these nanoparticles (NPs), the peptides are analyzed by MALDI-TOF-MS to obtain their mass fingerprints. Application of the method to a test library of 146 tryptic peptides showed that cationic MPCs/MMPCs target negatively charged peptides while the anionic MPCs/MMPCs target positively charged peptides. The extraction with these NPs is also accompanied by high concentration factors such they can be used to extract and concentrate microcystin-LR at levels below the WHO guideline of 1?g/L.
Bibliographical Information:

Advisor:

School:University of Massachusetts Amherst

School Location:USA - Massachusetts

Source Type:Master's Thesis

Keywords:analytical chemistry

ISBN:

Date of Publication:09/25/2007

© 2009 OpenThesis.org. All Rights Reserved.