Translational Control in Escherichia coli: Hfq and PvuII
Abstract (Summary)In bacteria which lack a nucleus, transcription and translation are concurrent and the majority of gene regulation occurs at the transcriptional level. However, regulating translation can yield very rapid responses, and there is increasing evidence of its role in bacteria. Hfq, an RNA-binding chaperone protein is a global regulator that binds the regulatory RNA DsrA and facilitates its interaction with rpoS mRNA. This interaction controls translation of rpoS, which specifies an important transcription factor. The present study explored the possibility that Hfq needs more than one binding site to make such interaction possible. Through mutational analysis of hfq, the in vivo interactions between Hfq, DsrA and rpoS mRNA were studied. The role of cis-acting translation control signals was also explored. The PvuII type II RM was studied to understand the role of translation control in a system requiring tight, time-dependent regulation. It was found that pvuIIC translation regulates the translation of pvuIIR and this control involves coupling.
School Location:USA - Ohio
Source Type:Master's Thesis
Keywords:translation hfq pvuii coupling c protein rpos
Date of Publication:01/01/2007