Synthesis of hepatitis B virus surface protein derivates in yeast S. cerevisiae
Hepatito B viruso paviršiaus baltym? darini? sintez? miel?se S. cerevisiae
HBV surface proteins PreS1[13–59]-S, PreS1[20–59]-S, PreS1[30–59]-S, PreS1[40–59]-S, PreS1[50–59]-S, PreS1[90–119]-S were produced in S.cerevisiae and purified. Electron microscopy suggested spherical virus-like particle formation for all the proteins except PreS1[90–119]-S. The PreS1[90–119] sequence was demonstrated to decrease protein solubility. Proteins are suitable for Tupaia primary hepatocyte binding investigations, diagnostic products and vaccine candidate development.
Source Type:Master's Thesis
Keywords:virusologija b virusinis hepatitas baltymai sintez? virology hepatitis proteins synthesis
Date of Publication:11/28/2007