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Subtipos e polimorfismo da região Pol do HIV-1, em pacientes imunizados com uma vacina terapêutica de células dendríticas

by de Melo, Valéria Oliveira

Abstract (Summary)
In this work we investigated the association of the HIV-1 subtypes with the clinical evolution of patients submitted to the dendritic cell (DC) therapeutic vaccine for HIV. Theautologous inactivated virus was used to pulse the DC which was subsequently used as a vaccine. Viral isolates of the patients were sequenced and their sequence used forfilogenetic analysis. To the clinical analysis the levels os TCD8+ and TCD4+ cells were followed for 1 year as well as the viral load (VL). From the 19 patients vaccinated 13showed subtype B, 5 subtype F and 1 subtype D. Among the patients with subtype F the VL had decreased 1,5 Log and while the subtype B patients the decrease was 1,16 Log.Regarding the immunological parameters the patients who controlled the VL also had a strong cellular response. However some patients who had an significant increase of cellular response at 112 days of immunization and partially controlled the VL (VL lt; 0,5) did not sustained this response and the virus returned at initial levels after 360 days. Due to small population we could not associate the virological response with the subtypes although there was a tendency of patients harbouring subtype F be able to show a better control of the virus after immunization. All patients of subtype F, but one, were the able to control the VL load for one year. Nevertheless, other factors besides the virus subtypes seem to be playing a role in driving a sustained response, such as innate immune and virus polymorphisms, activation of the antiviral state regulated by T reg lymphocytes among others not well known mechanisms
This document abstract is also available in Portuguese.
Bibliographical Information:

Advisor:Luiz Cláudio de Arraes Alencar

School:Universidade Federal de Pernambuco

School Location:Brazil

Source Type:Master's Thesis

Keywords:Clinical evolution HIV subtipes Dendritic cell

ISBN:

Date of Publication:11/09/2005

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