Study of immunogenicity of Neisseria lactamica antigens: use of monoclonal antibodies.
Immunological and epidemiological evidences suggest that the development of natural immunity to meningogoccal disease may be associated with crossreactive antigens together with Neisseria meningitidis and other commensal bacteria, like Neisseria lactamica. The present study aimed to investigate the immunogenicity of antigens of outer membrane vesicles (OMV) of N. lactamica with or without the presence of Bordetella pertussis (BP) used as an adjuvant. Groups of neonate BALB/c mice were immunized intranasally antigens of N. lactamica. The results of our studies showed the predominance of high titers of antibodies of IgG and IgM isotipes with high and intermediate avidity after intranasal immunization with N. lactamica. Immunoblot analysis of serum showed cross-reactivity proteins between the species of the gender Neisseria and the monoclonal antibodies used in this study. These results suggest that antigens of N. lactamica and N. meningitidis in common may be important in natural immunity against meningogoccal disease and in the development of vaccine.
Advisor:Elizabeth Natal de Gaspari; Sandro Rogerio de Almeida; Jorge Timenetsky; Elizabeth Natal de Gaspari
School:Universidade de São Paulo
Source Type:Master's Thesis
Keywords:Neisseria lactamica Antigens Monoclonal antibodies
Date of Publication:03/19/2008