Studies on the induction of acid phosphatase in response to phosphorus deficiency in Ulva lactuca L. (Ulvales, Cholrophyta)
The roles of phosphorus (P) starvation on the induction of intracellular acid phosphatase (ACP; EC 18.104.22.168) activity have been studied in a marine macroalga Ulva lactuca L. In comparison to creasy and dark green appearance in P-sufficient thalli (100 mM NaH2PO4), P-starved thalli (1 mM NaH2PO4) showed less crease and light green appearance. On exposure to 1 mM NaH2PO4, the growth rate, the contents of SRP, PP and P, and tissue C:P and N:P molar ratio decreased at day 3 and the contents of SNRP, TSP and polyphosphate decreased immediately. Intracellular ACP activity increased at day 3 after exposure to 1 mM NaH2PO4 and reached 16 folds of P-sufficient thalli at day 14, while extracellular alkaline phosphatase (AP; EC 22.214.171.124) activity increased at day 2 and reached the plateau after 4 days. Activity staining both on Native PAGE and IEF gel showed the induction of 10 and 9 ACP isoenzymes, respectively. Changes in intracellular ACP and extracellular AP activities were negatively correlated with SRP, SNRP, PP and P contents. After transferred to 100 mM NaH2PO4, the growth rate of 10 day-starvated thalli recovered after 5 days, the contents of SRP¡BSNRP¡BTSP and P, and the C:P and N:P molar ratio recovered to the level of P-sufficient thalli at day 1. When recovered to 100 mM NaH2PO4, extracellular AP activity of 10 day-starvated thalli decreased at day 2 and reached the minimum after day 8, while intracellular ACP activity decreased at day 3 and reached the minimum after day 8. The analog of Pi, Phi (1 mM) inhibited the intracellular ACP and extracellular AP activities induced by P swtarvation. The results of present investigation show that ACP has a role in the enhancement of P availability in U. lactuca via the enzymatically degradation of polyphosphates and organic P when suffers P deficiency.
Advisor:none; none; none; TM LEE
School:National Sun Yat-Sen University
School Location:China - Taiwan
Source Type:Master's Thesis
Keywords:acid phosphatase ulva lactuca phosphorus deficiency
Date of Publication:06/24/2003