Studies on Cytotoxic Secondary Metabolities of the Formosan Gorgonian Sinularia gibberosa and Isis hippuris.
In our continuing study on the chemical constituents of Taiwanese soft corals, the EtOAc extracts of a gorgonian coral Isis hippuris and a alcyonarian coral Sinularia gibberosa were investigated, respectively. Seven compounds, including 3£\,11£]-dihydroxy-24-methyl-22,25epoxy-5£\- furostan-18,20£]-lactone (1), 3-acetyl-2-deacetyl-22R-hippurin-1 (2), hippuristerone F (3), hippurin-1 (4), 22-epi-hippurin-1 (5), 3-acetyl-2- desacetyl-22-epi-hippurin-1 (6) and 2-desacetyl-22-epi-hippurin-1 (7) were isolated from I. hippuris. Three metabolites, 3£],11-dihydroxy-24- methylene-9,11-secocholest-5-en-9-one (8), 3£],11-dihydroxy-24-methyl-9, 11-secocholest-5-en- 9-one (9) and 3£]-hydroxy-11-acetoxy-24-methylene-9, 11-secocholest-5-en-9-one (10) were isolated from S. gibberosa. Among them, compounds 1¡V3, are new products. All metabolites 1¡V10 are steroids.
The structures of 1¡V10 were determined by physical and spectral analysis, including IR, MS, 1D NMR (1H, 13C) and 2D NMR ( 1H-1H COSY, HMQC, HMBC and NOESY ) and by comparison with the related physical and spectral data of the known compounds.
The cytotoxicity of the isolates against the P-388 ( mouse lymphocytic leukemia ), A-549 ( human lung adenocarcinoma ) and HT-29 ( human colon adenocarcinoma ) cancer cell lines were studied. Compound 9 and 10 showed significant cytotoxicity against P-388 cancer cell line. Metabolites 6 and 8 showed significant cytotoxicity against P-388 and HT-29 cancer cell lines. Compound 2, 4 and 5 exhibited potent cytotoxicity against the growth of P-388, A-549 and HT-29 cancer cell lines.
Advisor:J-N Lin; J-H Sheu; Y-G Wu
School:National Sun Yat-Sen University
School Location:China - Taiwan
Source Type:Master's Thesis
Keywords:sinularia gibberosa isis hippuris
Date of Publication:07/27/2001