Structure, enzymology and genetic engineering of Bacillus sp. RAPc8 nitrile hydratase.
Abstract (Summary)Microbial nitrile hydratases are important industrial enzymes that catalyse the conversion of nitriles to the corresponding amides. A thermostable, cobalt-type Bacillus sp. RAPc8 microbial nitrile hydratase was cloned and expressed in E.coli. In this study the primary aim was to determine the molecular structure of Bacillus sp. RAPc8 microbial nitrile hydratase.
School Location:South Africa
Source Type:Master's Thesis
Keywords:enzymes biotechnology industrial applications
Date of Publication:01/01/2005