Spatial resolution characterization of images taken from a capillary-based high pressure chamber for biological imaging studies [electronic resource] /
A capillary based high pressure chamber for biological fluorescence imaging is presented where a quartz capillary serves as both an optical window and the mechanical support. We demonstrate the pressure holding abilities of two capillaries of varying dimensions. We measure the point spread function of two capillaries and a flat slide for comparison. Spatial deconvolution uses the measured point spread function to restore images of microspheres. We find that there is significant spread laterally and axially in the measured point spread function for the 1.0mm OD x 0.8mm ID capillary and 1.0mm OD x 0.8mm ID capillary with a significant asymmetry in the lateral directions. We investigate image improvement laterally and axially after deconvolution with measured and calculated point spread functions. We find that the lateral resolution improves significantly with the use of deconvolution, however, neither the calculated nor the measured point spread functions systematically produce a better result.
School Location:USA - Ohio
Source Type:Master's Thesis
Keywords:fluorescence microscopy resolution optics spatial point spread function deconvolution high pressure chamber
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