Sources of variability in a proteomic experiment /
The study of proteomics holds the hope for detecting serious diseases earlier than is currently possible by analyzing blood samples in a mass spectrometer. Unfortunately, the statistics involved in comparing a control group to a diseased group are not trivial, and these difficulties have led others to incorrect decisions in the past. This paper considers a nested design that was used to quantify and identify the sources of variation in the mass spectrometer at BYU, so that correct conclusions can be drawn from blood samples analyzed in proteomics. Algorithms were developed which detect, align, correct, and cluster the peaks in this experiment. The variation in the m/z values as well as the variation in the intensities was studied, and the nested nature of the design allowed us to estimate the sources of that variation. The variation due to the machine components, including the mass spectrometer itself, was much greater than the variation in the preprocessing steps. This conclusion inspires future studies to investigate which part of the machine steps is causing the most variation.
School:Brigham Young University
School Location:USA - Utah
Source Type:Master's Thesis
Keywords:proteomics mass spectrometers multilevel models statistics variance components spectrometer
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