SDS-PAGE AND WESTERN BLOTTING ANALYSES OF INTERNALIN A IN LISTERIA MONOCYTOGENES AND LISTERIA SPP.
Attachment strength of Listeria spp. and their InlA expression was assessed. Listeria monocytogenes 19111 exhibited the strongest attachment strength with L. monocytogenes 19115, L. grayi, L. innocua, and L. monocytogenes 7644 being the weakest. InlA expression was not detected in silver stained SDS gels but was detected in Western blotting images. Internalin A was only detected in protein extracts of L. monocytogenes 19111 and 7644 with band intensities of 50.1 and 2.5 pixels, respectively. Greater InlA expression was correlated with higher attachment strength in L. monocytogenes 19111. Listeria monocytogenes 19115 did not express InlA but it had a stronger attachment than L. monocytogenes 7644 which demonstrated InlA expression. Intensity of InlA expressed in L. monocytogenes 19111 increased when temperature increased from 10 to 40 oC, and L. monocytogenes 7644 only expressed InlA at 40 oC. It was also determined that InlA was more expressed in nutrient-rich media than in nutrient-poor media.
Advisor:Charles H. White; Mark W. Schilling; William B. Mikel; Vance H. Watson; Charles H. White; Mark W. Schilling; William B. Mikel; Vance H. Watson; Charles H. White; Mark W. Schilling; William B. Mikel; Vance H. Watson; Juan L. Silva; Charles H. White
School:Mississippi State University
School Location:USA - Mississippi
Source Type:Master's Thesis
Keywords:food science nutrition and health promotion
Date of Publication:09/22/2006