Research of homologous antibodies anti-Borreliaburgdorferi in buffaloes (Bubalus bubalis) of Pará state, Brazil.
Rearing buffaloes in Brazil has been increasing notoriously and leaving of being a simpleactivity to justify the use of poor fertility lands. Right now the rearing buffalo is considered apromissory lucrative activity, but the information about sanitary aspects to improve buffaloeshealth are not consistent. Borreliosis is a systemic infectious disease caused by many Borreliaspecies, which have a cosmopolitan distribution, and affects various species of domestic andwild animals, including human beings. Borrelia theileri is the specie more commonlyreported in ruminants. However, these animals can also be infected by B. burgdorferi sensulato and B. coriaceae, which cause Lyme borreliosis and abortion epizootic bovine,respectively. Meanwhile, there are no seroepidemiologic studies of borreliosis in buffaloes.The aim of this study was to know the frequency of anti- B. burgdorferi homologousantibodies in buffaloes serum samples proceeding from Castanhal, Santa Isabel, NovaTimboteua and Santarém Novo, in the continental part of Pará state and Cachoeira do Arari inMarajó Island were collected. Serums of 491 buffaloes were analyzed by indirect ELISA test.The serologic analysis of the samples showed that 412 serums (83.91%) were positive,without different statistical significance between positive animals from Marajó Island81.469% (2362/284) and from the continental part 86.96% (180/207). Was observed thatbuffaloes of these five municipalities studied have high antibodies frequency againstspirochetes B. burgdorferi. The Correspondence Analysis test showed the formation of threedifferent municipalities groups of according with seropositives animals number. The firstgroup was formed by Cachoeira do Arari and Castanhal, the second by Nova Timboteua andSantarém Novo. The third group was constituted by Santa Isabel only, which presentedstatisticment loss antibodies frequency than others municipalities. The high frequency ofpositives animals found can be explained by presence of tick Boophilus microplus and by theexistence of report on Borrelia sp. infecting buffalo in the studied region. These facts suggestcross-reactivity between strain g39/40 of B. burgdorferi used as antigenic substratum andBorrelia species that infect buffaloes in Pará state. Despite of low specificity of indirectELISA test used in this study, it is a good method to select e screen infected animals in studiesabout Borrelia sp. in buffaloes.
Advisor:Adivaldo Henrique da Fonseca
Source Type:Master's Thesis
Keywords:borrelia búfalo elisa buffaloes medicina veterinaria
Date of Publication:02/27/2007