Replication from the primary origin of the mini-F plasmid
Abstract (Summary)Restricted Item. Print thesis available in the University of Auckland Library or available through Inter-Library Loan. The work in this thesis concerns the analysis of essential functions required for replication initiation from origin -1 of the mini-F replicon of F plasmid. The techniques principally employed were the cloning of restriction fragments, DNA sequencing and sequence analyses, assays for replicative ability in DNA polymerase 1 - dependent hosts, protein purification techniques and protein – DNA binding. This work led to the following findings - 1. A 198 base pair region of mini-F contains the sequence required for initiation of replication from origin 1 in a DNA polymerase 1 -dependent host. 2. The mini-F encoded proteins C, G and E are required for replication from this origin. 3. The C protein is a 38,739 dalton protein which binds in vitro to two DNA regions - one contains the operator of the C gene and the other region contains the minimal origin sequence.
School Location:New Zealand
Source Type:Master's Thesis
Date of Publication:01/01/1986