Details

RAPID DETECTION OF PROSTATE SPECIFIC ANTIGEN (PSA) ON A POLYMER LAB-ON-A CHIP

by THATI, SHILPA

Abstract (Summary)
In this work, a disposable protein biochip on a Cyclic-Olefin Copolymer (COC), which can be used for fast and easy detection of prostate specific antigen (PSA), has been designed and fabricated, and the method of detection of PSA varying within the concentration range of 4 ng to 200 ng has been successfully characterized with the help of this biochip. Traditionally prostate cancer is diagnosed by performing Digital Rectal Examination (DRE) or testing the PSA level in blood. The PSA levels in blood can be tested by various methods. But sample volume, time taken to get the results and contaminations are various problems in these methods. Numerous efforts have been made to detect the level of PSA at a micro scale, but most devices reported take 3 to 4 hours to give results and are very difficult to fabricate. So, in this research, a new approach with lab-on-a-chip has been developed which deals with all the above problems. The method developed in this work relies on the specificity of antibody-antigen interaction on polymer lab-on-a-chip. Sandwich immunoassay technique has been used, where the antigen to be detected is sandwiched between a primary and secondary antibody. This complex is then detected with the help of chemiluminescence. So a contamination free (due to the specificity of binding between an antigen and antibody), faster and cheaper detection of PSA has been successfully achieved. Disposable polymer lab-on-a-chips and low sample volume are added advantages in this method.
Bibliographical Information:

Advisor:

School:University of Cincinnati

School Location:USA - Ohio

Source Type:Master's Thesis

Keywords:prostate specific antigen polymer lab on a chip sandwich immunoassay

ISBN:

Date of Publication:01/01/2004

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