Q1U8S3 - a cousin to Majastridin

by Ottosson, Andreas

Abstract (Summary)

The aim of this work was to determine if the protein Majastridin found in the proteobacterium Rhodobacter blasticus has a functional relative in the hypothetical protein Q1U8S3/ B3XNV1 found in Lactobacillus reuteri. To be able to study the protein, it was overexpressed inĀ  E. coli-cells and purified. As a starting material, the L. reuteri Q1U8S3 gene previously cloned into a pET SUMO vector from Invitrogen was used. The produced protein will be a fusion protein containing a His6-tag, a SUMO-protein and the protein of interest. A nickel column in combination with a gel filtration column was used to purify the protein and after purification, crystallization experiments were set up using standardized kits.

Bibliographical Information:


School:Örebro universitet

School Location:Sweden

Source Type:Master's Thesis

Keywords:majastridin q1u8s3 biochemistry biokemi


Date of Publication:01/01/2009

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