Protein expression analysis of virulent and attenuated Leptospira interrogans.
Leptospirosis, one of the most spread zoonosis worldwide, is caused by bacteria of the genus Leptospira. Preventive measures are the best way to control the disease due to the difficulty to impair the proliferation of rodents and because no efficient vaccine is currently available. Functional genomics strategies have pointed a large number of proteins that could be important immunogens. This fact allied to published data reporting the identification of proteins involved in pathogenesis that are expressed only in virulent strains, led us to propose the use of proteomics as a tool to narrow down these studies. The methodology involved the preparation of protein extracts from tissuederived leptospires, separation by two-dimensional gel and identification of the spots by mass spectrometry. The central objective was to identify proteins expressed only in virulent bacteria. The identification of these proteins could help the search for proteins involved in virulence with a role during infection. Additionally, the data presented here represent a large step to clarify the biology and pathogenicity of leptospires, as well as the identification of potentially important vaccine candidates and/or proteins to compose more efficient diagnostic methods.
Advisor:Ana Lucia Tabet Oller do Nascimento; Eduardo Moraes Rego Reis; Maria Filomena de Andrade Rodrigues; Ana Lucia Tabet Oller do Nascimento
School:Universidade de São Paulo
Source Type:Master's Thesis
Keywords:Leptospira interrogans Bacterial pathogenicity Functional genomics Proteômica Proteomics
Date of Publication:07/10/2008