Production and evaluation of autogenous bacterin against streptococcal meningitis in swine
The present work was developed with the objective to determine the efficiency of an autogenous bacterin against Streptococcus suis serotype 2 and to test route and inoculation doses. Two experiments were accomplished with 64 animals in each experiment. In the Experiment I, 32 animals were vaccinated and 32 animals received placebo, being both groups challenged at the 60 days of age. In the Experiment II, the animals were divided in four groups: NVIP - 16 animals not vaccinated and challenged by the intraperitoneal route; VIP - 16 vaccinated animals and challenged by the intraperitoneal route; NVIV - 16 animals not vaccinated and challenged by the intravenous route; and VIV - 16 vaccinated animals and challenged by the intravenous route. All the groups were challenged at the 88 days of age. In the Experiment I, it was not possible to detectsignificant difference in the protection, clinical signs and lesions (Pgt;0.05) among the groups not vaccinated and vaccinated. However, the isolation of S. suis and the average of the bacterial count was higher (P lt;0.01) in the group not vaccinated. The results observed in Experiment II in the groups NVIV and VIV presented protection, clinical signs and lesions similar (Pgt;0.05), and the number of animals with positive isolation of S. suis and the average of the bacterial count was higher in the group NVIV (Plt;0.05). The groups NVIP and VIP presented significant difference for protection, clinical signs, lesions, number of positive isolations and average of the bacterial agent count (P lt;0.01). The intravenous challenge route was not appropriate for that experiment type and the intraperitoneal route presented favorable aspects. The vaccine was efficient for prevention of the infection with high virulent S. suis serotype 2, protectingthe animals with an efficiency of 87.5 percent.
Advisor:Paulo Sergio de Arruda Pinto; Paula Dias Bevilacqua; José Lúcio dos Santos; Walter Vieira Guimarães; José Eurico de Faria
School:Universidade Federal de Viçosa
Source Type:Master's Thesis
Date of Publication:09/05/2003