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Polimorfismos dos Genes das Citocinas IL-1alfa, IL -1beta, IL-10 e TNF-alfa

by Cordeiro, Cynthia Azeredo

Abstract (Summary)
Purpose: Toxoplasmic Retinochoroiditis (TR) is the most common identifiable cause of posterior uveitis in many parts of the world. There is a great controversy regarding which factors are responsible for the occurrence or reccurrence of TR. Experimental data have demonstrated a relevant role for IL-10, TNF-alfa, IL-1alfa and IL-1alfa in the modulation of acute ocular toxoplasmosis. Therefore, we aim to investigate thepossible association between an IL10 (-1082G/A), TNF-alfa (-308G/A), IL1A (-889C/T) and IL1B (+3954C/T) gene polymorphisms and TR in humans. Methods: One hundred patients with diagnosed TR were recruited from the Uveitis Section, Federal University of Minas Gerais. For comparison, one hundred healthy blood donors with positive serology for Toxoplasmosis and without retinal signs of previous TR were included in the study. Genomic DNA was obtained from oral swabs of individualsand amplified using polymerase chain reaction (PCR) with specific primers for each locus gene. PCR products were submitted to restriction endonuclease digestion and analyzed by polyacrylamide gel electrophoresis to distinguish specific alleles for eachgene, allowing the detection of the polymorphism and determination of genotypes.Results: In the IL10 gene polymorphism analysis, there was a significant difference in the genotype distribution between TR patients and control subjects (x² = 6.33, P = 0.04). Carriers of the IL10 -1082 A allele (AA + AG genotypes) were more often patients with TR than controls (x² = 5.97, P = 0.01, OR = 2.55, CI = 1.11This document abstract is also available in Portuguese.
Document Full Text
The full text for this document is available in Portuguese.
Bibliographical Information:

Advisor:Fernando Orefice; Antonio Lucio Teixeira Junior; Wesley Ribeiro Campos; Francisco Max Damico; Walderez Ornelas Dutra; Joyce Hisae Yamamoto

School:Universidade Federal de Minas Gerais

School Location:Brazil

Source Type:Master's Thesis

Keywords:Retina DeCS

ISBN:

Date of Publication:05/15/2008

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