Polimorfismo por ?Random Amplified Polymorphic DNA?(RAPD) em metacestódeos de Taenia soliumprovenientes de diferentes áreas geográficas do Brasile a reatividade de anticorpos IgG séricos de pacientescom neurocisticercose frente aos isolados obtidos

by da Costa, Ivanildes Solange

Abstract (Summary)
Neurocysticercosis (NC) is a polymorphic disease and the immune response inhuman carrier is heterogenic. In this study, 35 primers were used for amplifications byRandom Amplified Polymorphic DNA (RAPD) of Taenia solium metacestodes, from fivedifferent geographic areas in Brazil: 1) Distrito Federal (DF), Center West; 2) Barreiras(BA), Northeast and Southeast; 3) Hydro Basin of the Mosquito River (North of MinasGerais, RM-MG), 4) São Paulo (SP) and 5) Uberaba (Minas Gerais, UB-MG).Metacestodes saline crude extracts of four populations (DF, BA, RM-MG e SP) were usedfor the detection of specific IgG antibodies by ELISA and Western Blotting (WB). A totalof 157 serum samples of three groups, (G1): 49 NC patients; (G2): 68 patients with otherhelminthiasis: hydatidosis (10), taeniasis (20), strongyloidiasis (20), schistosomiasis (10)and hymenolepiasis (8); and (G3): 40 healthy individuals; were analyzed by ELISA. Fromthese, the 98 serum samples were assayed by WB; G1 (49), G2 (39) and G3 (10). Thegenetic distances, in disagreement percentage, between the metacestode populations werecalculated from of 15 RAPD markers and showed 49.5% (DF), 48% (BA), 38.5% (UBMG)and 28% (RM-MG and SP) of genetic distances. Six primers identified polymorphicfragments and the primers 26 (GGGTTTGGCA) and 29 (TCGCCAGCCA) allowed abetter differentiation of populations. The fragments of 1000, 500 and 326 pb (pairs ofbases) in the UB-MG and of 600 and 244 pb in RM-MG were amplified by primer 26. Thefragments generated by primer 29 were 500, 800 and 1191 pb, 300 and 1377 pb, 1000 pband 244 and 434 pb in SP, UB-MG, DF and BA populations, respectively. In G1, thepositivity by ELISA was: 90% (DF), 69% (BA), 71% (MG) and 67% (SP). The DF extractwas more antigenic than others (p=0.02). In WB, the 64-68 kDa antigens were recognizedin all extracts, exclusively, in serum samples from active NC patients (p=0.001). Variationin banding pattern was detected between the extracts (p<0.05). In G2, the serum samplesof hydatidosis patients presented from 70 to 90% positivity by ELISA in antigenicextracts (p<0.05); however, the bands recognition pattern in WB was different from thatpresented in G1 samples. The 77 kDa band was significantly identified in hydatidosissamples (p=0.0001). In conclusion, the T. solium populations analyzed showed geneticvariability and antigenic differences.
This document abstract is also available in Portuguese.
Bibliographical Information:

Advisor:Ana Maria Bonetti; Maria Aparecida de Souza; Luciana Pereira Silva; Fabiana Martins de Paula; Julia Maria Costa-Cruz

School:Universidade Federal de Uberlândia

School Location:Brazil

Source Type:Master's Thesis

Keywords:Neurocisticercose Taenia solium Neurocysticercosis Polymorphism Western Blotting


Date of Publication:04/07/2006

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