Papel de MIF (Fator de Inibição de Migração de Macrófagos) na proteção de células trofoblásticas (BeWo) e explantes de terceiro trimestre contra infecção por Toxoplasma gondii
Macrophage Migration Inhibitory Factor (MIF) performs various roles. It?s immunologicalfunctions include the modulation of the host response to pathogens. In contrast to other knowncytokines, MIF production is induced rather than suppressed by glucocorticoids, and MIF hasbeen found to override the immunosuppressive effects of glucocorticoids. The aim of thisstudy was to verify the role of MIF on the defense of trophoblastic cells (BeWo) ? like aexperimental model of first trimester of pregnancy and placental explants of third trimesteragainst infection by Toxoplasma gondii. For this purpose, BeWo cells were incubated inculture medium treated or not with STAg, rMIF, anti-MIF, supernatants of cultured cells(SPN) pre stimulated for MIF production, or SPN plus anti-MIF. Followed by infection orsham-infection with T. gondii the cells were again incubated in the presence of the samestimulus. Supernatants of BeWo cells were assessed for MIF production. BeWo cells wereprocessed to measure replication of parasites and index of infection and forimmunocitochemistry assay. In addition, placental explants were stimulated or not with STAgand infected or not with T. gondii. Explants were processed for morphologic andimmunohistochesmistry analyses and supernatants were accessed for MIF production. Resultsshowed that MIF is increased in supernatants of cultured BeWo cells infected with T. gondiior stimulated with STAg compared with non infected cells. However, it wasn´t observed forplacental explants. The index of infection and replication of parasites increased with blockadeof endogenous MIF and reduced in presence of exogenous MIF in BeWo cells. Thus, weconclude that MIF is an important factor in control of T. gondii infection in BeWotrophoblastics cells. The lack up regulation of MIF by infection in third trimester placentalexplants may have relationship with the more susceptible to infection at this gestational age.
Advisor:Neide Maria da Silva; Jose Roberto Mineo; Eloisa Amália Vieira Ferro; Sônia Maria Oliani
School:Universidade Federal de Uberlândia
Source Type:Master's Thesis
Date of Publication:07/30/2008