Origin and function of CD8 T cells in MHC class Ia-deficient mice [electronic resource] /

by Su, Jie.; Texas Southwestern, University of

Su, J., and J. Forman. CD8 T cells in MHC class Ia–deficient mice. AAI, Denver, CO, 2003. Su, J., R. E. Berg, S. Murry, and J. Forman. Thymus dependent MHC non class Ia selected memory phenotype CD8 T cells from naïve mice provide rapid protection against infection. AAI, San Diego, CA, 2005. xiii List of Figures 1. An elevated percentage of CD8 T cells in DKO are CD8?+CD8?-………………………….50 2. There are less CD8??CD44hi cells and similar CD8?? cells in DKO mice compared to B6 mice…………………………………………………………………………………………..52 3. A significant portion of CD8?? T cells in DKO mice is CD44hiCD122+Ly6C+ and CD62Llo……………………………………………………………………………...............53 4. Expression of NK cell markers by CD8??CD44hi cells from naïve B6 and DKO mice ………………………………………………………………………………………………..55 5. Co-expression of CD94 and NKG2A or NKG2D by CD8??+CD44hi cells from naïve B6 and DKO mice…………………………………………………………………………….....57 6. Paucity of CD8+CD4- T cells in the thymus of DKO mice……………………………….....58 7. The thymic emigration rate of CD8?? cells and is reduced in DKO mice………………….60 8. Most CD8?? RTE in both B6 and DKO mice are CD44lo……………………………….....62 9. Most CD4 RTE in both B6 and DKO mice are CD44lo………………………………….....63 10. Most CD8?? cells in DKO mice are thymus-dependent…………………………................64 11. Most thymus independent CD8?? CD44hi cells in DKO mice are CD44hi…………………65 12. Lower proliferation rate of CD8?+CD44hi and CD8?+CD44lo T cells in DKO mice ……………………………………………………………………………………................67 13. High apoptosis rate of CD8?? T cells in DKO mice………………………………………..70 14. Homeostasis-driven proliferation of CD8 T cells in class Ia-deficient recipients…………..71 15. Homeostasis-driven proliferation of B6 CD8 T cells in recipients lacking a single class Ia molecule…………………………………………………………………..............................73 xiv 16. Homeostasis-driven proliferation of CD8 T cells from normal mice in class Ia-deficient recipients……………………………………………………………………………………74 17. Extensive proliferation of CD8??CD44hi cells after in vitro anti-CD3 culture…...............78 18. Rapid IFN-? secretion by CD8??CD44hi cells after in vitro anti-CD3 stimulation………..79 19. Thymus dependent CD8?? cells expand and acquire CD44hi expression in response to LM challenge…………………………………………………………………………………….81 20. CD8?? cells from Txbm animals do not respond to LM challenge………………...............82 21. CD8?? cells from Txbm animals do not mount an antigen specific response to LM challenge………………………………………………………………………….................84 22. Transferred purified CD8??CD44hi T cells from naive DKO mice were detected in recipient mice after LM challenge……………………………………………………………………..85 23. CD8??CD44hi T cells from naive DKO mice show an antigen-specific response to LM……………………………………………………………………………………………87 24. CD8??CD44hi T cells from naive DKO mice show an H2-M3 restricted antigen-specific response to LM……………………………………………………………………………....88 25. CD8??CD44hi cells from DKO mice secrete IFN-? after anti-CD3 administration in vivo………………………………………………………………………………..................90 26. IFN-? secreting CD8??CD44hi cells from DKO mice after anti-CD3 administration in vivo are not skewed forwards a particular V? expressing TCR…………………………………..91 27. Proliferation of CD8??CD44hi cells in response to IL-2 stimulation……………………….93 28. CD8??CD44hi cells from DKO mice secrete IFN-? in response to IL-12 and IL-18 stimulation……………………………………………………………………..…………….94 xv 29. No CTL responses were detected against target cells expressing NKG2D ligands by CD8+CD44hi cells from naïve DKO mice…………………………………………………..96 30. Model……………………………………………………………………………………….111 xvi xvii List of Abbrevations The following list does not include standard abbreviation published in the first issue of each volume of The Journal of Immunology bm bone marrow ?2m ?2-microglobulin DC dendritic cell DKO B6.H-2Kb-/-Db-/- ER endoplasmic reticulum GATA-3 GATA-binding protein-3 HKLM heat killed LM HSP heat shock protein ICS intracellular cytokine staining IFNAR type I IFN?/? receptor iIEL intestinal intraepithelial lymphocytes IL-7R? interleukin 7 receptor ?-chain LCMV lymphocytic choriomeningitis virus LLO Listeriolysin O LM Listeria monocytogenes