Organization and Evolution of the CYP2ABFGST Gene Cluster in Rat, and a Comparison with Human and Mouse
The evolution of gene families can be best understood by studying the modern organization and functions of family members, and by comparing parallel families in different species. As an interesting example of gene family evolution, the CYP2ABFGST gene cluster has been characterized in rat. Eighteen loci from six subfamilies have been identified by sequencing PCR-amplified exons or introns from BAC clones. The overall organization of the CYP2ABFGST cluster in rat is much more similar to the mouse than to the human cluster. The rat cluster is the simplest of the three, with genes from each subfamily in tandem. Specific loci from all three species have been extensively compared to sort out relationships between genes. These comparisons allow us to reconstruct the probable structure of the CYP2ABFGST cluster in the common ancestor of primates and rodents, and to infer a model of the evolution of this gene cluster in the three species.
School Location:USA - Ohio
Source Type:Master's Thesis
Keywords:cyp2abfgst gene cluster molecular evolution rat
Date of Publication:01/01/2005