A NEW POLYMER LAB-ON-A-CHIP FOR POLYMERASE CHAIN REACTION (PCR) USING NON-CONTACT INFRARED THERMOCYCLES
In this work, a new polymer Lab-on-a-chip for polymerase chain reaction (PCR) has been developed and characterized using infrared (IR) based thermocycles. These new polymer PCR chips are made by Polycarbonate (PC) and Cyclic Olefin Copolymer (COC). Both of them are designed to have a microchannel for externally inserting a thermocouple to detect temperature during thermocycling. So, these polymer PCR chips can be controlled without using an on-chip integrated temperature sensor and heater, which allows the polymer PCR chips to be a fully disposable platform for low cost PCR chips. The infrared (IR) based heating method also has advantages from not only the non-contact heating but also the fast heating and cooling times. Although PC and COC polymers have a low thermal conductivity compared with Si and glass, the heat energy attained from infrared lamp is enough to successfully perform the thermocycles for PCR. The entire system consists of an IR heating source, an externally insertable thermocouple, a signal conditioning circuit, and Labview control system. The IR heating source is a commercial tungsten halogen lamp which operates at regulated brightness levels for controlling a constant temperature during dwell times and turned off for cooling step. Cooling is accelerated by an air microfan. The overall control of the system including signal conditioning is performed by a Labview program through GPIB cable. In order to obtain the result of PCR amplification from the developed polymer chips, the effective annealing temperature of primer sets and BSA concentration in PCR cocktails on PCR has been explored and optimized.. As a result, these polymer PCR chips achieve the successful amplifications of 195 and 544 base pair of ë phase DNA. These polymer PCR chips can be immediately used for PCR amplification and also can be applied for constructing a whole system for performing consecutive biochemical processes such as DNA sampling, purification, amplification, and then analysis. With these approaches, a fully disposable polymer PCR chip with the sampling and analysis capabilities of DNA will be feasible.
School:University of Cincinnati
School Location:USA - Ohio
Source Type:Master's Thesis
Keywords:pcr polymer infrared mems
Date of Publication:01/01/2005