Myogenic regulatory factors expression in rat diaphragm muscle with heart failure

by Silva Lopes, Francis da

Abstract (Summary)
Heart failure (HF) is characterized bya reduced tolerance to exercise due to early fatigue and dyspnea. Alterations that may contribute to dyspnea in this syndrome are diaphragmatic myopathy with atrophy and shift from type 11 "fast" to type I "slow" myosin heavy chain (MHC). However, the skeletal muscle-specific molecular regulatory mechanisms that alter MHC expression in the diaphragm during heart failure have not been defined. The purpose of this investigation was to determine whether myosin heavy chain expression during heart failure is associated with changes in myogenic regulatory factors (MRFs) mRNA expression in Wistar rat diaphragm with heart failure (HF) induced by monocrotaline. Costal diaphragm (DIA) muscle from HF (n=09) and control (n=06) 3-4 week old, 80-100g male Wistar rats was studied when overt HF had developed in the HF animais 22 days after monocrotaline administration. MyoD and myogenin expression were determined by RT-PCR, MHC isoform expression was determined by polyacrylamide gel electrophoresis. HF animais presented decreased myosin heavy chain lIa/llx protein isoform and MyoD gene expression content, without altering MHC I & IIb and myogenin expression. In summary, our results show that in HF, alterations in MyoD mRNA expression may, in part, explain alterations in MHC lIalllx content
This document abstract is also available in Portuguese.
Bibliographical Information:

Advisor:Maeli Dal Pai Silva; Maeli Dal Pai Silva [Orientador]; Marina Politi Okoshi; Maria Alice da Cruz Hofling

School:Universidade Estadual de Campinas

School Location:Brazil

Source Type:Master's Thesis

Keywords:Myogenic regulatory factors Heart failure Diaphragm


Date of Publication:09/01/2005

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