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Multi-Enzyme Biocatalysis Using Nano-Structured Materials for Bioprocessing Applications

by El-Zahab, Bilal Mohamad

Abstract (Summary)
With the increasing awareness of environmental safety and the need for renewable fuels, enzyme-catalyzed reactions provide convenient substitutes for future industries relative to most catalysis used today. To our date, multienzyme systems involving cofactors have not been fully explored. In this work, novel cofactor-dependent multienzyme biocatalysts were developed for bioprocessing applications. Areas investigated ranging from proof-of-concept to specific applications were: 1) The separation of enzymes from bacteria: to mimic microbes, multienzyme extracts from trichloroethylene degrading bacteria were separated and in vitro degradation was achieved. 2) Immobilization of a multi-enzyme system in nanoporous glass: successful enzyme-cofactor interaction was possible on the same surface if concave structures were used while smaller pores and longer spacers provided remarkable enhancement. 3) Attachment to nanoparticles: efficient cofactor regeneration and reuse for the novel sequential enzyme-catalyzed synthesis of methanol and L-lactate from CO2. Theoretical and experimental approaches to optimize the synthesis involved studying the flexibility of the polymeric spacer-arm over a range of solubility conditions. In summary, a green multi-enzyme system able to catalyze complex redox reactions was demonstrated while using a cofactor regeneration mechanism rendering it cost-effective. Such system suggests a wealth of potential in catalysis, remediation, and sensing applications.
Bibliographical Information:

Advisor:

School:The University of Akron

School Location:USA - Ohio

Source Type:Master's Thesis

Keywords:biocatalysis nanoparticles multi enzyme cofactor regeneration immobilization

ISBN:

Date of Publication:06/09/2009

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