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Monitoring Chain Dynamics by Luminescence Using a Long-Lived Ruthenium Dye

by Quinn, Cristina

Abstract (Summary)
The purpose of this research project was to determine the feasibility of labelling a water-soluble polymer with a water-soluble dye and quencher. The water-soluble dye chosen was bis-(2,2-bipyridine)-ruthenium(II)

-5-amino-1,10-phenanthroline hexafluorophosphate, RuNH2 , with a water

solubility of 1×10^?3 mol/L. 3,5-Dinitrobenzyl alcohol was found to be an efficient quencher

with a quenching rate constant of 2.7×10^9 M^?1 s^?1 as well as a water-solubility of 5×10^?3 mol/L. Both the dye and quencher were modified in a way such that they could be covalently linked to a polymer. RuNH2 was converted to bis-(2,2-bipyridine)-ruthenium(II)-5-isothiocyanato-1,10-

phenanthroline hexafluoro-phosphate, RuNCS, using thiophosgene to yield an active isothiocyanate group. 3,5-Dinitrobenzylamine, DNB-NH2 , was synthesized via tritylamination of the commercially available 3,5-dinitrobenzyl chloride.

A synthetic pathway has been established to covalently attach the dye and quencher to poly(N,N-dimethylacrylamide)(PDMA). Luminescence of this system was first characterized in N,N-dimethylformamide

(DMF) rather than water to allow for future comparisons to be made between this water-soluble system and the previously established non-water-soluble system. Luminescence analysis of the RuNCS labelled polymers in DMF could be fitted with a sum of three exponentials with the strongest contribution being that of a 1000 ns long-lived species which is characteristic of the free dye. A luminescence decay of a polymer labelled with both RuNCS and DNB-NH2 was acquired and showed static quenching of the ruthenium dye by the quencher.

Bibliographical Information:

Advisor:

School:University of Waterloo

School Location:Canada - Ontario

Source Type:Master's Thesis

Keywords:luminescence polymer dynamics ruthenium pdma chemistry

ISBN:

Date of Publication:01/01/2006

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