Mikroverkapselung von humanen Parathyreozyten mit Natriumcellulosesulfat und Poly-DADMAC in der Behandlung des postoperativen Hypoparathyreoidismus
Due to the various metabolic functions of parathyroid hormone satisfactory treatment of permanent hypoparathyroidism after thyroid and parathyroid surgery remains difficult. Previous trials with allotransplantation of human parathyroid tissue failed because of rejection. Besides their immunoissolation abilities semi-permeable membranes of microcapsules ensure access to nutritients or oxygen and transfer of peptide hormones. An established human parathyroid cell culture model was used to examine 30 cryopreserved specimens from patients with secondary hyperparathyroidism. We analysed PTH secretion and function before and after microencapsulation with sodiumcellulose sulphate (NaCS) and polydiallyldimethylammonium chloride (Poly-DADMAC). Cell viability of cryopreserved tissue in culture increased from 72(11,7% to 95(4,3% after two days. Physiological responsiveness to calcium suppression was still intact and comparable to the encapsulated group. Mean capsule diameter was 0,33(0,02mm, while pore size was 1,39(0,04nm. In comparison to the group of non-encapsulated cells the PTH-secretion increased in the group of the encapsulated cells approximately linear. This study shows that microencapsulation with NACS and Poly-DADMAC allows nutritive supply and endocrine secretion of human parathyroid cells.
School:Humboldt-Universität zu Berlin
Source Type:Master's Thesis
Date of Publication:01/06/2005