MICROWAVE DETECTION OF GOLD NANOPARTICLES IN POLYMER FILMS
Abstract (Summary)This work explored the possibility of coupling of an immunoassay-based detection with microwave-based sensing in an effort to improve the overall speed of the microbial agent detection. More specifically, we attempt to improve the speed of detection of antibody-antigen binding by coupling antibodies labeled with gold nanoparticles (approximately 20 nm in diameter) and using a microwave cavity to rapidly detect them. Gold-tagged antibodies were immobilized in polyvinyldene fluoride (PVDF) membrane filters at concentrations ranging from 100 fg/L to 1 µg/L. A cylindrical microwave cavity was used to measure perturbations in the 10 to 36 GHz range due to presence of nanoparticles inside the PVDF filters. A model of the cavity and the PVDF filters was composed in FEMLAB to predict perturbations inside the resonant cavity.
School:University of Cincinnati
School Location:USA - Ohio
Source Type:Master's Thesis
Date of Publication:01/01/2005