Mastite bovina causada por Prototheca zopfii
The bovine mastitis due to Prototheca zopfii has economic, social and public health importance, because it causes persistent infection, refractive to traditional therapeutic procedures, reduces significantly the milk production, besides to be considered zoonosis. This research had as objective the isolation of Prototheca spp. in bulk tanks, milk cans, cows with mastitis and herd environment, and to determine mastitis predisposing factors as well. Prototheca spp. was isolated in 10/81 composed samples of bulk tanks. Predisposing factors to Prototheca spp. were analysed in 13 milk herds from Paraná and São Paulo States. In these herds, 383 cows were examined, both Hostein breed and hybrid, and samples were collected from water, soil, free stall bedding material, swabs of teat cup rubbers, cattle, calves and swine manure. Prototheca spp. was cultured in Sabouraud?s agar and characterized according to cultural aspects and microscopic morphology. Out of the 13 herds studied, ten had Prototheca spp. isolated. In eight, the agent was isolated in milk samples of the bulk tanks, but only in three herds Prototheca spp. was isolated from cows and the environment. Out of the total of 383 cows examined, Prototheca spp. was isolated in 20 (5.2%) cows with mastitis, and 18 of them were characterized as P. zopfii. In four herds Prototheca spp. was isolated in the water of the drinking trough, supply, sewage and water puddled in the stable and milking parlor, from samples of samples of teat cup rubbers and manure from calves and swines. P. zopfii was identified in samples of cow pen soil, teat cup rubbers and manure of calves and swines. Through univaried analysis, predisposing factors associated with mastitis due to P. zopfii were: pasture system, pasture and silage feed, milking in stable, permanence of cows in the lounge without food after milking, raising of swines near bovine housing, presence of dogs, cats and rodents, milk storage in bulk tanks, equipment sanitation with hot water, use of alkaline detergent and acids, lack of hygienization of the teats with water, pre-immersion of the teats in small cups with antiseptic reflux, laboratorial diagnosis and antibiogram, utilization of vaccine against mastitis from bacterial origin, calves? feeding with clinical mastitis milk and the Hostein breed.
Advisor:Ernst Eckehardt Müller; Ernst Eckehardt Müller [Orientador].; Jane Megid; Márcio Garcia Ribeiro
Source Type:Master's Thesis
Date of Publication:08/03/2006