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MBG - Induced EMT

by Nadour, Alaa M

Abstract (Summary)
Interest and research in Epithelial-mesenchymal transition are currently growing at a high level due the importance of this process in embryogenesis, organ development, tumor progression, and several pathologic conditions in adult tissues. Epithelial to mesenchymal transformation (EMT) is now believed to play a major role in the pathogenesis of renal interstitial fibrosis, and organ fibrosis in general. This unique event along with other several cellular pathways, leads to excessive accumulation and deposition of extracelular matrix components that cause fibrous scars and destruction of kidney parenchyma, with consequent end-stage renal failure.Multiple intracellular signaling pathways are implicated in the regulation of this complicated event. Marinobufagenin (MBG), a cardiotonic steroid, and an inhibitor of the enzymatic activity of Na/K-ATPase, has shown a potency to activate multiple signaling pathways that ultimately leads to EMT in renal epithelial cells. Therefore, it was reasonable to hypothesize that elevated levels of this cardiotonic steroid could be a potent inducer of EMT. Our study on the porcine proximal epithelial cell line demonstrates that Marinobufagenin induces EMT in time and concentration dependent fashion.
Bibliographical Information:

Advisor:

School:University of Toledo Health Science Campus

School Location:USA - Ohio

Source Type:Master's Thesis

Keywords:

ISBN:

Date of Publication:01/01/2007

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