Laboratory study of the wound healing response to epithelial scrape injury in the human cornea

by Júnior, Renato Ambrósio

Abstract (Summary)
Purpose: To examine the early wound healing response to epithelial scrape in human corneas. Methods: Normal corneas had epithelial scrape prior to enucleation. Histology, TUNEL assay, Ki67, SMA and transmission electron microscopy were performed. Results: Epithelial scrape was performed in six corneas from ½ to 65 hours prior to preservation. Keratocyte apoptosis was detected in the anterior stroma in all scraped corneas. Keratocyte proliferation was detected exclusively 65 hours after scrape. No myofibroblast was detected. One cornea was not scraped (control). Conclusion: Results obtained in human corneas (keratocyte apoptosis and proliferation) were similar to animal models
This document abstract is also available in Portuguese.
Bibliographical Information:

Advisor:Milton Ruiz Alves; Newton Kara Jose; Arlindo José Freire Portes; Eduardo Melani Rocha; Marcos Wilson Sampaio; Walter Yukihiko Takahashi; Newton Kara Jose

School:Universidade de São Paulo

School Location:Brazil

Source Type:Master's Thesis

Keywords:APOPTOSIS CORNEAL STROMA/cytology EPITHELIUM CORNEAL/anatomy & histology CORNEAL/growth development CORNEAL/surgery IMMUNOHISTOCHEMISTRY/methods MICROSCOPY CONFOCAL/methods ELECTRON/methods FLUORESCENCE/methods MITOSIS/physiology


Date of Publication:05/19/2004

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