Kinetic isotope effect in the [beta]-subunit mechanism of tryptophan synthase ; excited state tautomerization of azaindole
Abstract (Summary)In Chapter I of this thesis, the ?-subunit mechanism of tryptophan synthase is investigated. We explored the addition of indole to the preformed aminoacrylate L- serine-enzyme complex to form an indolenine quinonoid intermediate. A kinetic isotope effect (KIE) is observed in the tautomerization of the indolenine quinonoid intermediate, and the KIE is dependent on the presence of allosteric ligands.
School:The University of Georgia
School Location:USA - Georgia
Source Type:Master's Thesis
Date of Publication: