Isolamento, caracterização química e avaliação da propriedade inseticida do óleo essencial de Piper amplum Kunth

by Adams Angnes, Sandra Inês

Abstract (Summary)
The Piperaceae family belongs to the superorder Nymphaeiflorae and order Piperales, comprising 5 main genera with 1400 species. Piper and Peperomia are the most important genera with about 700 and 600 species, respectively. The genus Piper provides several representatives, which are essential oil producers. Theessential oils find application as raw materials for different chemical industrial sectors. In natura they may also show cytotoxic, insecticidal and organoleptic properties, which increases their scientific and technological potential. The present work describes the extraction, isolation, identification and properties of the chemical constituents from the aereal parts of Piper amplum Kunth, ocurring in the ParqueNatural Municipal São Francisco de Assis, Blumenau em Santa Catarina. The essential oil obtained from the leaves and fruits were extracted by hidrodestillation using a modified Clevenger apparatus, providing maximum yields of 0,12 e 0,045%,respectively. The chemical composition of the leaves essential oil was determined by a combination of analytical techniques, namely gas chromatography (GC), gas chromatography coupled to mass spectrometry (GC-ME) and retention index (RI). The latest technique used as standards a homologous series of linear hydrocarbons. It was identified 19 compounds, comprising 90% of the essential oil constituents, among them a-pinene (12,9%); limonene (3,8%); a-copaene (4,2%); E-cariophylene (24,4%); valencene (16,3%) e a-cadinol (8,5%). It was also observed a strong seasonal influence on the monoterpene hydrocarbons concentration, which reacheda maximum value during the summer season (January). The insecticide property of the leaves essential oil against Cx. Quinquefasciatus gave an unexpected negative result. The extracts from green and mature fruits were obtained by continuous extraction in Soxhlet apparatus employing the binary system n-hexane and ethyl acetate (1:1). Although their chemical composition was shown to be the same, theextract yield from mature fruits was much superior, 2,18%, when compared to 0,78% for the green fruits. It was isolated from the fruit extract by column chromatography a major constituent, which was partially characterised. Conventional and DEPT13C and 1H Nuclear Magnetic Resonance technique indicated the compound to have a parafinic chain associated to a phenolic system. Complementary analysis is requiredfor the full structural characterization. For a better evaluation of the plant therapeutic properties, further studies are suggested
This document abstract is also available in Portuguese.
Bibliographical Information:

Advisor:Moacir Geraldo Pizzolatti; Flávia Aparecida Fernandes da Rosa; Iêda Maria Begnini; Ricardo Andrade Rebelo

School:Universidade Regional de Blumenau

School Location:Brazil

Source Type:Master's Thesis

Keywords:familia Piperaceae Piper amplum Kunth piperaceae family essential oils


Date of Publication:12/09/2005

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