INFRARED MICROSPECTROSCOPIC TECHNIQUES FOR THE QUALITATIVE ANALYSIS OF CROSS-SECTIONED RENAL CALCULI AND EMBEDDED MINERALIZED DEPOSITS
This thesis presents investigations into the qualitative analysis of renal stones and embedded deposits using infrared (IR) microspectroscopy. The incorporation of a microscope into infrared analysis improves spatial resolution, allowing the interrogation of small areas of interest, and the identification of microscopic particles. The use of IR reflection/absorption (R/A) techniques as a means to identify mineral deposits embedded in tissue is discussed, as is the use of IR reflectance off the surface of cross-sectioned calculi. These techniques have been found to be more accurate and less time-consuming than the traditional practices of contrast staining, especially for samples containing multiple components. In an attempt to quantify a particular component, attenuated total internal reflection (ATR) IR microspectroscopy calibration curves were created, against which unknown samples were measured. However, more work is needed for this method to be considered truly quantitative. Finally, a spectral library of common renal stone components is presented.
School Location:USA - Ohio
Source Type:Master's Thesis
Keywords:infrared microspectroscopy renal stones calculi imaging attenuated total interan reflectance specular diffuse
Date of Publication:01/01/2004