In vitro Charakterisierung autologer Aktivierung und Klonierung von T-Lymphozyten aus psoriatischen Plaques
Psoriasis is a complex inflammatory disease of the skin characterized by a dense infiltration of T-lymphocytes and a hyperproliferation of the epithelial layer. A host of experimental and clinical data suggest that psoriasis is a T cell mediated disorder. The nature of T-cell-stimulating antigens is still unknown. One way to identify putative antigen(s) is the definition of T-cell-receptor specifities using randomized combinatorial peptide libraries. This requires the isolation and expansion of T cell clones from psoriatic plaques in vitro. Therefore we established a gamma-interferon Elispot-assay which allows quantification of the frequency of activated plaque-derived T cells in vitro. In addition, we show that activated T cell clones can be sorted via CD25 and cloned. The expanded clones can also be restimulated by autologous cells. Our results should be useful in the design of experiments aiming at a systematic analysis of the specifity of T cell clones present in psoriatic plaques.
School:Humboldt-Universität zu Berlin
Source Type:Master's Thesis
Keywords:Psoriasis Interferon-gamma psoriasis t cells gamma-Interferon CD25-positive YF 3404 3414 3415
Date of Publication:07/13/2005