Implication des gènes de réparation de l'ADN dans la stabilité du génome d'Arabidopsis thaliana - Étude de l'instabilité des microsatellites
The present study allowed us to develop a reporter system based on a bacterial gene encoding for ?-glucuronidase (GUS), in order to measure microsatellite instability in Arabidopsis thaliana. Microsatellites are particular regions of the genome undergoing frequent mutations during replication. A correlation between length of the repetitive tracts and microsatellite instability was demonstrated. Besides, the orientation of the microsatellite influenced its instability. In two later studies, we have also studied the effect of mismatch repair genes (MMR) on microsatellite instability. Mismatch repair system corrects errors that occur during the replication of microsatellites. We showed that inactivation of AtPMS1 and AtMSH2 genes increased tracts instability, hence the importance of these genes in the maintenance of microsatellite stability and genome stability in general.
School Location:Canada - Quebec / Québec
Source Type:Master's Thesis
Date of Publication:04/01/2005